Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas )

Type Article
Date 2022-09
Language English
Author(s) Sorée MarionORCID1, 2, Delavat François3, Lambert Christophe4, Lozach Solen5, Papin Mathias6, Petton BrunoORCID5, Passerini DelphineORCID1, Dégremont LionelORCID7, Hervio Heath DominiqueORCID5
Affiliation(s) 1 : Ifremer, MASAE, F‐44311 Nantes, France
2 : Ifremer, SGMM, F‐29280 Plouzané, France
3 : Nantes Université, CNRS, US2B, UMR6286 F‐44000 Nantes, France
4 : Univ Brest, CNRS, Ifremer, IRD, LEMAR, F‐29280 Plouzané, France
5 : Univ Brest, Ifremer, CNRS, IRD, LEMAR, F‐29280 Plouzané, France
6 : Ifremer, EMMA, F‐85230 Bouin, France
7 : Ifremer, ASIM, F‐17390 La Tremblade, France
Source Environmental Microbiology (1462-2912) (Wiley), 2022-09 , Vol. 24 , N. 9 , P. 4401-4410
DOI 10.1111/1462-2920.15996
WOS© Times Cited 1
Note Double Special Issue: Microbiomes and Microbial Communities, and Pathogen and Antimicrobial Resistance Ecology
Abstract

Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V. parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V. parahaemolyticus accumulation and depuration in adult Crassostrea gigas, we developed a GFP-tagged V. parahaemolyticus strain (IFVp201-gfp+), as well as a rapid and efficient quantification method in C. gigas oysters haemolymph by flow cytometry. Impact of the life-history of C. gigas on accumulation and depuration of V. parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V. parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates.

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Figure S1. Experimental protocols with IFVp201-gfp+ for the preliminary assay in February 2021 (A) and the challenge test with naive and environmental oysters in October 2021 (B). 291 KB Open access
Publisher's official version 10 699 KB Open access
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How to cite 

Sorée Marion, Delavat François, Lambert Christophe, Lozach Solen, Papin Mathias, Petton Bruno, Passerini Delphine, Dégremont Lionel, Hervio Heath Dominique (2022). Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ). Environmental Microbiology, 24(9), 4401-4410. Publisher's official version : https://doi.org/10.1111/1462-2920.15996 , Open Access version : https://archimer.ifremer.fr/doc/00763/87507/