Natural Variation in Physicochemical Profiles and Bacterial Communities Associated withAedes aegyptiBreeding Sites and Larvae on Guadeloupe and French Guiana

Type Article
Date 2021-01
Language English
Author(s) Hery Lyza1, Guidez Amandine2, Durand Audrey-Anne3, Delannay Christelle1, Normandeux-Guimond Josiann3, Reynaud Yann1, Issaly Jean2, Goindin Daniella1, Legrave Gregory4, Gustave Joel5, Raffestin Stephanie6, Breurec Sebastien7, 8, 9, Constant Philippe3, Dusfour Isabelle2, Guertin Claude3, Vega-Rua Anubis1
Affiliation(s) 1 : Inst Pasteur Guadeloupe, Transmiss Reservoir & Pathogens Divers Unit, Lab Vector Control Res, Morne Joliviere, Guadeloupe, France.
2 : Inst Pasteur French Guiana, Vector Control & Adaptat Unit, Vectopole Amazonien Emile Abonnenc, Cayenne, French Guiana, France.
3 : Ctr Amand Frappier Sante Biotechnol, INRS, Laval, PQ, Canada.
4 : Inst Pasteur Guadeloupe, Lab Environm & REG HLTH AGCY GUADELOUPEFood Hyg, Morne Joliviere, Guadeloupe, France.
5 : Reg Hlth Agcy Guadeloupe, Gourbeyre, Guadeloupe, France.
6 : Inst Pasteur French Guiana, Lab Environm & Hyg, Cayenne, French Guiana, France.
7 : Inst Pasteur Guadeloupe, Transmiss Reservoir & Divers Pathogens Unit, Pointe A Pitre, Guadeloupe, France.
8 : Univ Antilles, Hyacinthe Bastaraud Fac Med, Pointe A Pitre, Guadeloupe, France.
9 : INSERM, Ctr Clin Invest 1424, Pointe A Pitre, Les Abymes, France.
Source Microbial Ecology (0095-3628) (Springer), 2021-01 , Vol. 81 , N. 1 , P. 93-109
DOI 10.1007/s00248-020-01544-3
WOS© Times Cited 11
Keyword(s) Aedes aegypti, Breeding site, Physicochemical parameter, Bacterial community
Abstract

Aedes aegyptidevelop in aquatic habitats in which mosquito larvae are exposed to physicochemical elements and microorganisms that may influence their life cycle and their ability to transmit arboviruses. Little is known about the natural bacterial communities associated withA. aegyptior their relation to the biotic and abiotic characteristics of their aquatic habitats. We characterized the physicochemical properties and bacterial microbiota ofA. aegyptibreeding sites and larvae on Guadeloupe and in French Guiana. In addition, we explored whether geographic location, the type of breeding site and physicochemical parameters influenced the microbiota associated with this mosquito species. We used large-scale 16S rRNA gene sequencing of 160 breeding sites and 147 pools ofA. aegyptilarvae and recorded 12 physicochemical parameters at the sampled breeding sites. Ordination plots and multiple linear regression were used to assess the influence of environmental factors on the bacterial microbiota of water and larvae. We found territory-specific differences in physicochemical properties (dissolved oxygen, conductivity) and the composition of bacterial communities inA. aegyptibreeding sites that influenced the relative abundance of several bacteria genera (e.g.,Methylobacterium,Roseoccocus) on the corresponding larvae. A significant fraction of the bacterial communities identified on larvae, dominated byHerbiconiuxandMicrovirgagenera, were consistently enriched in mosquitoes regardless the location. In conclusion, territory-specific differences observed in the biotic and abiotic properties ofA. aegyptibreeding sites raise concern about the impact of these changes on pathogen transmission by differentA. aegyptipopulations.

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Publisher's official version 17 3 MB Open access
ESM 1 Rarefaction curves of OTU diversity for each sample. Each sample contains nearly 20, 000 sequences to ensure equal sampling depth 1 1 MB Open access
ESM 2 Mean values for bacterial diversity and richness indices associated with water at breeding sites and A. aegypti larvae. 1 49 KB Open access
ESM 3 Relative abundance of the most abundant phyla. Bar shows mean relative abundance of the bacterial taxa sequenced from A. aegypti larvae and water samples from French Guiana and Guadeloupe. 1 1 MB Open access
ESM 4 Relative abundance of the most abundant genera. Bar shows mean relative abundance of the bacterial taxa sequenced from A. aegypti larvae and water samples from French Guiana and Guadeloupe 1 1 MB Open access
ESM 5 Spatial structuration and variability of microbiota associated with water at breeding sites and A. aegypti larvae. Non-metric multidimensional scaling plots based on UniFrac distances for... 1 240 KB Open access
ESM 6 Significant differences on bacteria genera abundance according to sample type (water vs. larval samples) and/or the territory 56 KB Open access
ESM 7 Canonical correlation analysis of 12 physicochemical variables and the 100 most abundant bacteria genera in Ae. aegypti breeding sites water (a) and larvae (b). COD, chemical oxygen demand... 33 KB Open access
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Hery Lyza, Guidez Amandine, Durand Audrey-Anne, Delannay Christelle, Normandeux-Guimond Josiann, Reynaud Yann, Issaly Jean, Goindin Daniella, Legrave Gregory, Gustave Joel, Raffestin Stephanie, Breurec Sebastien, Constant Philippe, Dusfour Isabelle, Guertin Claude, Vega-Rua Anubis (2021). Natural Variation in Physicochemical Profiles and Bacterial Communities Associated withAedes aegyptiBreeding Sites and Larvae on Guadeloupe and French Guiana. Microbial Ecology, 81(1), 93-109. Publisher's official version : https://doi.org/10.1007/s00248-020-01544-3 , Open Access version : https://archimer.ifremer.fr/doc/00771/88291/