Environmental microplastics disrupt swimming activity in acute exposure in Danio rerio larvae and reduce growth and reproduction success in chronic exposure in D. rerio and Oryzias melastigma

Type Article
Date 2022-09
Language English
Author(s) Cormier BettieORCID1, 2, Cachot Jerome1, Blanc Melanie2, 4, Cabar Mathieu4, Clérandeau Christelle1, Dubocq FlorianORCID2, Le Bihanic Florane1, Morin Bénédicte1, Zapata Sarah1, Bégout Marie-LaureORCID3, Cousin XavierORCID4
Affiliation(s) 1 : Bordeaux University, EPOC, UMR CNRS 5805, Avenue des Facultés, 33400, Talence, France
2 : Man-Technology-Environment Research Centre, School of Science and Technology, Örebro University, Fakultetsgatan 1, 701 82, Örebro, Sweden
3 : MARBEC, Univ. Montpellier, CNRS, Ifremer, IRD, INRAE, 34250, Palavas-les-flots, France
4 : MARBEC, Univ. Montpellier, CNRS, Ifremer, IRD, INRAE, 34250, Palavas-les-flots, France
Source Environmental Pollution (0269-7491) (Elsevier BV), 2022-09 , Vol. 308 , P. 119721 (10p.)
DOI 10.1016/j.envpol.2022.119721
Keyword(s) Environmental microplastics, Fish, Reprotoxicity, Growth and behaviour alterations, Biomarkers, Adsorbed chemicals
Abstract

Microplastics (MPs), widely present in aquatic ecosystems, can be ingested by numerous organisms, but their toxicity remains poorly understood. Toxicity of environmental MPs from 2 beaches located on the Guadeloupe archipelago, Marie Galante (MG) and Petit-Bourg (PB) located near the North Atlantic gyre, was evaluated. A first experiment consisted in exposing early life stages of zebrafish (Danio rerio) to MPs at 1 or 10 mg/L. The exposure of early life stages to particles in water induced no toxic effects except a decrease in larval swimming activity for both MPs exposures (MG or PB). Then, a second experiment was performed as a chronic feeding exposure over 4 months, using a freshwater fish species, zebrafish, and a marine fish species, marine medaka (Oryzias melastigma). Fish were fed with food supplemented with environmentally relevant concentrations (1% wet weight of MPs in food) of environmental MPs from both sites. Chronic feeding exposure led to growth alterations in both species exposed to either MG or PB MPs but were more pronounced in marine medaka. Ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities were only altered for marine medaka. Reproductive outputs were modified following PB exposure with a 70 and 42% decrease for zebrafish and marine medaka, respectively. Offspring of both species (F1 generation) were reared to evaluate toxicity following parental exposure on unexposed larvae. For zebrafish offspring, it revealed premature mortality after parental MG exposure and parental PB exposure produced behavioural disruptions with hyperactivity of F1 unexposed larvae. This was not observed in marine medaka offspring. This study highlights the ecotoxicological consequences of short and long-term exposures to environmental microplastics relevant to coastal marine areas, which represent essential habitats for a wide range of aquatic organisms.

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Cormier Bettie, Cachot Jerome, Blanc Melanie, Cabar Mathieu, Clérandeau Christelle, Dubocq Florian, Le Bihanic Florane, Morin Bénédicte, Zapata Sarah, Bégout Marie-Laure, Cousin Xavier (2022). Environmental microplastics disrupt swimming activity in acute exposure in Danio rerio larvae and reduce growth and reproduction success in chronic exposure in D. rerio and Oryzias melastigma. Environmental Pollution, 308, 119721 (10p.). Publisher's official version : https://doi.org/10.1016/j.envpol.2022.119721 , Open Access version : https://archimer.ifremer.fr/doc/00782/89436/