A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment

Type Article
Date 2022-07
Language English
Author(s) Bernard Clément1, Locard-Paulet MarieORCID2, Noël CyrilORCID3, Duchateau MagalieORCID4, Giai Gianetto Quentin4, 5, Moumen Bouziane1, Rattei ThomasORCID6, Hechard Yann1, Jensen Lars JuhlORCID2, Matondo MarietteORCID4, Samba-Louaka AscelORCID1
Affiliation(s) 1 : Laboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS, 7267, Poitiers, France
2 : Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark
3 : IFREMER-IRSI-Service de Bioinformatique (SeBiMER), Centre Bretagne, Plouzane, France
4 : Institut Pasteur, Université de Paris, Proteomics Platform, Mass Spectrometry for Biology Unit, UAR2024, CNRS 2000, Paris, France
5 : Institut Pasteur, Université de Paris, Department of Computation Biology, Bioinformatics and Biostatistics Hub, Paris, France
6 : Centre for Microbiology and Environmental Systems Science; Doctoral School Microbiology and Environmental Science, University of Vienna, Vienna, Austria
Source Nature Communications (2041-1723) (Springer Science and Business Media LLC), 2022-07 , Vol. 13 , N. 1 , P. 4104 (14p.)
DOI 10.1038/s41467-022-31832-0
WOS© Times Cited 8
Abstract

Encystment is a common stress response of most protists, including free-living amoebae. Cyst formation protects the amoebae from eradication and can increase virulence of the bacteria they harbor. Here, we mapped the global molecular changes that occur in the facultatively pathogenic amoeba Acanthamoeba castellanii during the early steps of the poorly understood process of encystment. By performing transcriptomic, proteomic, and phosphoproteomic experiments during encystment, we identified more than 150,000 previously undescribed transcripts and thousands of protein sequences absent from the reference genome. These results provide molecular details to the regulation of expected biological processes, such as cell proliferation shutdown, and reveal new insights such as a rapid phospho-regulation of sites involved in cytoskeleton remodeling and translation regulation. This work constitutes the first time-resolved molecular atlas of an encysting organism and a useful resource for further investigation of amoebae encystment to allow for a better control of pathogenic amoebae.

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Publisher's official version 14 3 MB Open access
Supplementary Information 12 8 MB Open access
Peer Review File 12 246 KB Open access
Description of Additional Supplementary Information 14 KB Open access
Supplementary Data 1 4 MB Open access
Supplementary Data 2 34 KB Open access
Supplementary Data 3 17 MB Open access
Supplementary Data 4 10 MB Open access
Supplementary Data 5 7 MB Open access
Supplementary Data 6 16 KB Open access
Supplementary Data 7 14 KB Open access
Reporting Summary 3 1 MB Open access
Source Data 36 KB Open access
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Bernard Clément, Locard-Paulet Marie, Noël Cyril, Duchateau Magalie, Giai Gianetto Quentin, Moumen Bouziane, Rattei Thomas, Hechard Yann, Jensen Lars Juhl, Matondo Mariette, Samba-Louaka Ascel (2022). A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment. Nature Communications, 13(1), 4104 (14p.). Publisher's official version : https://doi.org/10.1038/s41467-022-31832-0 , Open Access version : https://archimer.ifremer.fr/doc/00785/89743/