Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
Type | Article | ||||||||||||
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Date | 2022-08 | ||||||||||||
Language | English | ||||||||||||
Author(s) | de La Broise Denis1, Ventura Mariana1, Chauchat Luc3, Guerreiro Maurean1, Michez Teo1, Vinet Thibaud2, Gautron Nicolas1, Le Grand Fabienne3, Bideau Antoine1, Goïc Nelly Le1, Bidault Adeline1, Lambert Christophe3, Soudant Philippe3 | ||||||||||||
Affiliation(s) | 1 : CNRS, IRD, Ifremer, Université de Bretagne Occidentale, UMR6539 LEMAR, F-29280 Plouzané, France 2 : DENITRAL, F-22400 Lamballe, France 3 : CNRS, IRD, Ifremer, Université de Bretagne Occidentale, UMR6539 LEMAR, F-29280 Plouzané, France |
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Source | Marine Drugs (1660-3397) (MDPI AG), 2022-08 , Vol. 20 , N. 8 , P. 499 (19p.) | ||||||||||||
DOI | 10.3390/md20080499 | ||||||||||||
WOS© Times Cited | 4 | ||||||||||||
Note | This article belongs to the Special Issue Marine Thraustochytrids: Biology, Chemical Ecology and Biotechnology | ||||||||||||
Keyword(s) | Thraustochytrids, Aurantiochytrium, PUFA, DHA, digestate, oxygen, scale-up, pilot | ||||||||||||
Abstract | The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA–rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production costs, this study addresses the feasibility of the non-axenic (non-sterile) cultivation of Aurantiochytrium mangrovei on industrial substrates (as nitrogen and mineral sources and glucose syrup as carbon and energy sources), and its scale-up from laboratory (250 mL) to 500 L cultures. Pilot-scale reactors were airlift cylinders. Batch and fed-batch cultures were tested. Cultures over 38 to 62 h achieved a dry cell weight productivity of 3.3 to 5.5 g.L−1.day−1, and a substrate to biomass yield of up to 0.3. DHA productivity ranged from 10 to 0.18 mg.L−1.day−1. Biomass productivity appears linearly related to oxygen transfer rate. Bacterial contamination of cultures was low enough to avoid impacts on fatty acid composition of the biomass. A specific work on microbial risks assessment (in supplementary files) showed that the biomass can be securely used as feed. However, to date, there is a law void in EU legislation regarding the recycling of nitrogen from digestate from animal waste for microalgae biomass and its usage in animal feed. Overall, the proposed process appears similar to the industrial yeast production process (non-axenic heterotrophic process, dissolved oxygen supply limiting growth, similar cell size). Such similarity could help in further industrial developments. |
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