Genotype x nutrition interactions in European sea bass (Dicentrarchus labrax): Effects on gut health and intestinal microbiota
|Author(s)||Torrecillas S.1, Rimoldi S.1, 2, Montero D.1, Serradell A.1, Acosta F.1, Fontanillas R.3, Allal Francois4, Haffray P.5, Bajek A.6, Terova G.2|
|Affiliation(s)||1 : Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain
2 : Department of Biotechnology and Life Sciences, University of Insubria, Via J.H. Dunant, 3, 21100 Varese, Italy
3 : Skretting Aquaculture Research Centre, Stavanger, Norway
4 : MARBEC, University of Montpellier, CNRS, Ifremer, IRD, 34250 Palavas-les-Flots, France
5 : SYSAAF (French Poultry and Aquaculture Breeders Technical Centre), 35042 Rennes, France
6 : Ecloserie Marine de Graveline Ichtus, Route des Enrochements, 59820, Gravelines, France
|Source||Aquaculture (0044-8486) (Elsevier BV), 2023-09 , Vol. 574 , P. 739639 (22p.)|
|WOS© Times Cited||1|
|Keyword(s)||Aquaculture, Future diets, GALT, Gut morphology, Microbiota|
One of the main objectives of the present study was an effective replacement of dietary fishmeal/fish oil (FM/FO) by new raw materials without negatively affecting European sea bass (Dicentrarchus labrax) performance and health status within a selection breeding context. The genomic selection of this species is still in its infancy if compared to salmonids and in particular, the role of D.labrax genome in shaping the gut microbiome, has been scarcely investigated.
Wildtype (WT) and selected (GS) sea bass were fed with two diets: a control (20% FM/ 5–9% FO), and a “future” (F) diet, in which FM was decreased to 10% being replaced by poultry meal, whereas FO was completely replaced by a blend of rapeseed, poultry, and algae oils.
The morphological evaluation of the intestine revealed a well-organized folding pattern and a conserved gut epithelial barrier for all fish groups. Despite a basal level of inflammation in the proximal intestine of WT fish, no differences were observed neither in the morphometric characteristics of goblet cells nor in the expression of GALT-related genes in response to fish genotype or diet. At distal intestine, WT fish showed a higher inflammatory status and larger goblet cells than GS fish and within the same genotype, fish fed the F diet had in general larger goblet cells. In distal intestine, a significant effect was found on the expression of 3 out of 7 target GALT genes. In particular, the expression of cytokines il-1β, tnf-α, and il-10 was different, showing an interaction effect diet x genotype.