Rotavirus VLP2/6: a new tool for tracking rotavirus in the marine environment

Type Article
Date 2004-09
Language English
Author(s) Loisy Fabienne, Atmar Robert L., Cohen Jean, Bosch Albert, Le Guyader Soizick
Affiliation(s) IFREMER, Microbiol Lab, F-44311 Nantes 3, France.
Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA.
CNRS, Unite Mixte, INRA, F-91198 Gif Sur Yvette, France.
Univ Barcelona, Dept Microbiol, Enter Virus Grp, E-08028 Barcelona, Spain.
Source Research in Microbiology (0923-2508) (Elsevier), 2004-09 , Vol. 155 , N. 7 , P. 575-578
DOI 10.1016/j.resmic.2004.04.002
WOS© Times Cited 10
Keyword(s) Stability, Tracer, Seawater, Virus like particles, Rotavirus
Abstract The potential of rotavirus 2/6-virus-like-particles (VLP2/6) for use as tracers in the marine environment was investigated. The stability of bovine rotavirus (strain RF) and VLP2/6 in natural seawater at 25degreesC for six days was studied. ELISA and western blot methods were used to quantify the particles. The rates of decline of rotavirus particles and VLP2/6 were similar (approximately 0.5 log 10 per day). Western blot analysis showed that the integrity of capsid proteins VP2 and VP6 was conserved during the incubation time. These results demonstrate that VLP2/6 particles have the same stability in seawater as rotavirus particles. Thus, VLP2/6 can be used as a tracer, which should be of particular value for studying the fate of rotavirus particles in the marine environment.
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