Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia
|Author(s)||Lopez Flores L1, Suarez Santiago V2, Longet D3, Saulnier Denis1, Chollet Bruno1, Arzul Isabelle1|
|Affiliation(s)||1 : IFREMER, Lab Genet & Pathol, Stn Tremblade, F-17390 La Tremblade, France.
2 : Univ Granada, Fac Sci, Dept Bot, E-18071 Granada, Spain.
3 : Univ Geneva, Dept Zool & Anim Biol, CH-1211 Geneva, Switzerland.
|Source||Parasitology (0031-1820) (Cambridge University Press), 2007-12 , Vol. 134 , N. 14 , P. 1941-1948|
|WOS© Times Cited||17|
|Keyword(s)||Molecular phylogeny, Actin gene, Ostrea edulis parasite, Bonamia ostreae|
|Abstract||Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and demonstrated that the paralogous actin genes found in Bonamia resulted from a duplication of the original actin gene after the Bonamia origin.|