Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei

Type Article
Date 2010-11
Language English
Author(s) Labreuche Yannick1, 4, Veloso Artur1, De La Vega Enrique1, Gross Paul S.2, Chapman Robert W.3, Browdy Craig L.3, Warr Gregory W.1, 2
Affiliation(s) 1 : Med Univ S Carolina, Marine Biomed & Environm Sci Ctr, Charleston, SC 29412 USA.
2 : Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA.
3 : Marine Resources Inst, S Carolina Dept Nat Resources, Charleston, SC 29412 USA.
4 : IFREMER, Département Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie, BP 2059, 98846 Nouméa cedex, New Caledonia
Source Developmental And Comparative Immunology (0145-305X) (Elsevier Sci Ltd), 2010-11 , Vol. 34 , N. 11 , P. 1209-1218
DOI 10.1016/j.dci.2010.06.017
WOS© Times Cited 63
Keyword(s) Shrimp, Litopenaeus vannamei, RNAi, dsRNA, Argonaute, Sid-1, Antiviral immunity
Abstract Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both RNAi-related and innate antiviral responses. They also suggest that these two mechanisms of antiviral response may activate the same pathway, requiring Lv-Sid 1 and Lv-Ago 2 induction. (C) 2010 Elsevier Ltd. All rights reserved.
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Labreuche Yannick, Veloso Artur, De La Vega Enrique, Gross Paul S., Chapman Robert W., Browdy Craig L., Warr Gregory W. (2010). Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei. Developmental And Comparative Immunology, 34(11), 1209-1218. Publisher's official version : https://doi.org/10.1016/j.dci.2010.06.017 , Open Access version : https://archimer.ifremer.fr/doc/00015/12652/