Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix

Type Article
Date 2016-06
Language English
Author(s) Landreau M.1, 2, 3, Duthoit Frederique1, 2, 3, Claeys-Bruno M.4, Vandenabeele-Trambouze O.1, 2, 3, Aubry T.5, Godfroy AnneORCID1, 2, 3, Le Blay Gwenaelle1, 2, 3
Affiliation(s) 1 : Univ Bretagne Occidentale, IUEM, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France.
2 : IFREMER, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France.
3 : CNRS, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France.
4 : Aix Marseille Univ, LISA EA4672, Marseille, France.
5 : UFR Sci & Tech, LIMATB, Lab Ingn Mat Bretagne, Equipe Rheol, Brest, France.
Source Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2016-06 , Vol. 120 , N. 6 , P. 1531-1541
DOI 10.1111/jam.13118
WOS© Times Cited 3
Keyword(s) (hyper)thermophilic marine micro-organisms, anaerobiosis, entrapment, gellan, immobilization, xanthan
Abstract Aims

The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures.
Methods and Results

The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect on cell viability and a good growth within and outside the beads.
Conclusion

This suggests that entrapment in a gellan–xanthan matrix could be employed for the culture of anaerobic (hyper)thermophilic marine micro-organisms.
Significance and Impact of the Study

(Hyper)thermophilic marine micro-organisms possess a high biotechnological potential. Generally microbial cells are grown as free-cell cultures. The use of immobilized cells may offer several advantages such as protection against phage attack, high cell biomass and better production rate of desired metabolites.
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How to cite 

Landreau M., Duthoit Frederique, Claeys-Bruno M., Vandenabeele-Trambouze O., Aubry T., Godfroy Anne, Le Blay Gwenaelle (2016). Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix. Journal Of Applied Microbiology, 120(6), 1531-1541. Publisher's official version : https://doi.org/10.1111/jam.13118 , Open Access version : https://archimer.ifremer.fr/doc/00332/44352/