Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic

Type Article
Date 2021-12
Language English
Author(s) Wang Seaver1, Tang Weiyi2, Delage Erwan3, Gifford Scott4, Whitby Hannah5, González Aridane G.6, 7, Eveillard Damien3, Planquette Helene7, Cassar Nicolas1, 7
Affiliation(s) 1 : Division of Earth and Ocean Sciences, Duke University, Grainger Environment Hall, 9 Circuit Drive, Box 90328, Durham, NC, 27708, USA
2 : Department of Geosciences, Princeton University, Princeton, NJ, USA
3 : LS2N, UMR 6004, CNRS, Université de Nantes, 44000, Nantes, France
4 : Department of Marine Sciences, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
5 : Department of Earth, Ocean, and Ecological Sciences, School of Environmental Sciences, University of Liverpool, Liverpool, UK
6 : Instituto de Oceanografía y Cambio Global (IOCAG), Universidad de Las Palmas de Gran Canaria, ULPGC, Las Palmas, Spain
7 : Laboratoire des Sciences de l’Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), Technopôle Brest-Iroise, 13 Plouzané, 29280, Locmaria-Plouzané, France
Source Scientific Reports (2045-2322) (Springer Science and Business Media LLC), 2021-12 , Vol. 11 , N. 1 , P. 5508 (14p.)
DOI 10.1038/s41598-021-84969-1
Keyword(s) Element cycles, Marine biology
Abstract

Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N2) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N2 fixation and net community production (NCP). Maximum N2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae. Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N2 fixation coastal sites.

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How to cite 

Wang Seaver, Tang Weiyi, Delage Erwan, Gifford Scott, Whitby Hannah, González Aridane G., Eveillard Damien, Planquette Helene, Cassar Nicolas (2021). Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic. Scientific Reports, 11(1), 5508 (14p.). Publisher's official version : https://doi.org/10.1038/s41598-021-84969-1 , Open Access version : https://archimer.ifremer.fr/doc/00685/79677/