Total mineralization of 2-ethylhexyl nitrate by bacterial cocultures
Type | Article | ||||||||||||
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Date | 2010-11 | ||||||||||||
Language | English | ||||||||||||
Author(s) | Nicolau Elodie1, 2, Della Giustina Gabriela1, Jouanneau Yves2, Marchal Remy1 | ||||||||||||
Affiliation(s) | 1 : Department of Biotechnology, Institut Français du Pétrole (IFP), 1-4 Avenue de Bois Préau, 92852, Rueil-Malmaison, France 2 : CEA, IRTSV, LCBM, Grenoble, France |
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Source | World Journal Of Microbiology & Biotechnology (0959-3993) (Springer), 2010-11 , Vol. 26 , N. 11 , P. 2113-2116 | ||||||||||||
DOI | 10.1007/s11274-010-0395-6 | ||||||||||||
Keyword(s) | 2-EHN, Biodegradability, Cocultures, Cetane index | ||||||||||||
Abstract | 2-Ethylhexyl nitrate (2-EHN) is a widely-used chemical which is commonly added to diesel oil to boost its cetane index. The 2-EHN molecule is recalcitrant to biodegradation but still utilized as sole carbon source by Mycobacterium austroafricanum IFP 2173. The incomplete degradation of 2-EHN by this strain results in the accumulation of an intermediary metabolite i.e. 4-ethyldihydrofuran-2(3H)-one (4-EDF). The study aimed at isolating 4-EDF degraders in order to achieve total mineralization of 2-EHN in cocultures with M. austroafricanum IFP 2173. Bacterial isolates were obtained from diesel-contaminated soil by enrichment in serial cultures supplemented with 4-EDF, the degradation of which was monitored by CO2 measurements. Two strains were isolated and identified as Bacillus cereus and Burkholderia sp., respectively. Complete mineralization of 2-EHN was achieved by associating M. austroafricanum IFP 2173 with either bacterial isolate in cocultures. In the context of environmental acceptability, efficient degradation of a potentially persistent pollutant by a bacterial consortium is demonstrated. |
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