Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus

Type Article
Date 2021-07
Language English
Author(s) Slobodkina Galina1, Allioux Maxime4, Merkel Alexander1, Cambon Bonavita Marie-AnneORCID2, Alain Karine3, Jebbar Mohamed4, Slobodkin Alexander1
Affiliation(s) 1 : Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia
2 : Laboratoire de Microbiologie des Environnements Extrêmes LM2E, Univ Brest, CNRS, IFREMER, IRP 1211 MicrobSea, UMR 6197, Plouzané, France
3 : Laboratoire de Microbiologie des Environnements Extrêmes LM2E, Univ Brest, CNRS, IFREMER, IRP 1211 MicrobSea, UMR 6197, Plouzané, France
4 : Laboratoire de Microbiologie des Environnements Extrêmes LM2E, Univ Brest, CNRS, IFREMER, IRP 1211 MicrobSea, UMR 6197, Plouzané, France
Source Frontiers In Microbiology (1664-302X) (Frontiers Media), 2021-07 , Vol. 12 , P. 679245 (15p.)
DOI 10.3389/fmicb.2021.679245
Keyword(s) sulfate reduction, chemolithoautotroph, anaerobe, hyperthermophile, Archaea
Abstract

Hyperthermophilic archaea of the genus Archaeoglobus are the subject of many fundamental and biotechnological researches. Despite their significance, the class Archaeoglobi is currently represented by only eight species obtained as axenic cultures and taxonomically characterized. Here, we report the isolation and characterization of a new species of Archaeoglobus from a deep-sea hydrothermal vent (Mid-Atlantic Ridge, TAG) for which the name Archaeoglobus neptunius sp. nov. is proposed. The type strain is SE56T (=DSM 110954T = VKM B-3474T). The cells of the novel isolate are motile irregular cocci growing at 50–85°C, pH 5.5–7.5, and NaCl concentrations of 1.5–4.5% (w/v). Strain SE56T grows lithoautotrophically with H2 as an electron donor, sulfite or thiosulfate as an electron acceptor, and CO2/HCO3− as a carbon source. It is also capable of chemoorganotrophic growth by reduction of sulfate, sulfite, or thiosulfate. The genome of the new isolate consists of a 2,115,826 bp chromosome with an overall G + C content of 46.0 mol%. The whole-genome annotation confirms the key metabolic features of the novel isolate demonstrated experimentally. Genome contains a complete set of genes involved in CO2 fixation via reductive acetyl-CoA pathway, gluconeogenesis, hydrogen and fatty acids oxidation, sulfate reduction, and flagellar motility. The phylogenomic reconstruction based on 122 conserved single-copy archaeal proteins supported by average nucleotide identity (ANI), average amino acid identity (AAI), and alignment fraction (AF) values, indicates a polyphyletic origin of the species currently included into the genus Archaeoglobus, warranting its reclassification.

Full Text
File Pages Size Access
Publisher's official version 15 4 MB Open access
Presentation 58 KB Open access
Table 1 41 KB Open access
Table 2 16 KB Open access
Top of the page

How to cite 

Slobodkina Galina, Allioux Maxime, Merkel Alexander, Cambon Bonavita Marie-Anne, Alain Karine, Jebbar Mohamed, Slobodkin Alexander (2021). Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus. Frontiers In Microbiology, 12, 679245 (15p.). Publisher's official version : https://doi.org/10.3389/fmicb.2021.679245 , Open Access version : https://archimer.ifremer.fr/doc/00705/81715/