Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification

Type Article
Date 2023-01
Language English
Author(s) Coelho Márcio A. G.ORCID1, 2, Pearson Gareth A.ORCID1, Boavida Joana R. H.ORCID3, Paulo DiogoORCID1, Aurelle DidierORCID3, 4, Arnaud‐haond SophieORCID5, Gómez‐gras DanielORCID6, 7, 8, Bensoussan Nathaniel3, 9, López‐sendino PaulaORCID9, Cerrano CarloORCID10, 11, 12, 13, Kipson SilvijaORCID14, 15, Bakran‐petricioli TatjanaORCID14, Ferretti ElianaORCID16, 17, Linares CristinaORCID7, 8, Garrabou JoaquimORCID3, 9, Serrão Ester A.ORCID1, 18, Ledoux Jean‐baptisteORCID19
Affiliation(s) 1 : Centre for Marine Sciences (CCMAR) University of Algarve Faro ,Portugal
2 : MARE – Marine and Environmental Sciences Centre ISPA‐Instituto Universitário Lisboa,Portugal
3 : Aix Marseille Univ., Université de Toulon, CNRS, IRD, MIO Marseille, France
4 : Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum National d'Histoire Naturelle, CNRS Sorbonne Université Paris ,France
5 : MARBEC (Marine Biodiversity, Exploitation and Conservation) Univ. Montpellier, IFREMER, CNRS, IRD Sète Cedex ,France
6 : Hawai‘i Institute of Marine Biology University of Hawai‘i at Mānoa Kaneohe Hawaii, USA
7 : Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals Universitat de Barcelona (UB) Barcelona, Spain
8 : Institut de Recerca de la Biodiversitat (IRBio) Universitat de Barcelona (UB) Barcelona ,Spain
9 : Departament de Biologia Marina Institut de Ciències del Mar (CSIC) Barcelona, Spain
10 : Dipartimento di Scienze della Vita e dell’Ambiente (DiSVA) Università Politecnica delle Marche Ancona ,Italy
11 : Consorzio Nazionale Interuniversitario per le Scienze del Mare (CoNISMa) Rome, Italy
12 : Stazione Zoologica Anton Dohrn, Department of Integrative Marine Ecology Ischia Marine Centre Naples, Italy
13 : Fano Marine Center Fano ,Italy
14 : Department of Biology, Faculty of Science University of Zagreb Zagreb ,Croatia
15 : SEAFAN – Marine Research & Consultancy Zagreb, Croatia
16 : Studio Associato GAIA s.n.c. Genoa, Italy
17 : Institute of Marine Science The University of Auckland Auckland ,New Zealand
18 : CIBIO/InBIO‐Centro de Investigação em Biodiversidade e Recursos Genéticos Vairão ,Portugal
19 : CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental Universidade do Porto Porto ,Portugal
Source Ecology And Evolution (2045-7758) (Wiley), 2023-01 , Vol. 13 , N. 1 , P. e9740 (21p.)
DOI 10.1002/ece3.9740
WOS© Times Cited 5
Keyword(s) Atlantic-Mediterranean transition, cryptic diversity, incomplete lineage sorting, Octocorallia, phylotranscriptomics, species delimitation

The accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA-seq derived single-copy orthologues [SCO] and mt-mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species-tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocorals.

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Coelho Márcio A. G., Pearson Gareth A., Boavida Joana R. H., Paulo Diogo, Aurelle Didier, Arnaud‐haond Sophie, Gómez‐gras Daniel, Bensoussan Nathaniel, López‐sendino Paula, Cerrano Carlo, Kipson Silvija, Bakran‐petricioli Tatjana, Ferretti Eliana, Linares Cristina, Garrabou Joaquim, Serrão Ester A., Ledoux Jean‐baptiste (2023). Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification. Ecology And Evolution, 13(1), e9740 (21p.). Publisher's official version : , Open Access version :