The ZooScan and the ZooCAM zooplankton imaging systems are intercomparable: A benchmark on the Bay of Biscay zooplankton

Type Article
Date 2023-11
Language English
Author(s) Grandremy NinaORCID1, Dupuy Christine2, Petitgas PierreORCID3, Le Mestre Sophie4, Bourriau Paul1, Nowaczyk Antoine5, Forest Bertrand6, Romagnan Jean-Baptiste1
Affiliation(s) 1 : DECOD (Ecosystem Dynamics and Sustainability), IFREMER, INRAE, Institut Agro, Centre Atlantique, Rue de l’Ile d’Yeu - BP 21105, Nantes, 44311, France
2 : BIOFEEL, UMRi LIENSs, La Rochelle Université/CNRS, La Rochelle, France
3 : IFREMER, RBE, Centre Atlantique, Rue de l’Ile d’Yeu, Nantes, France
4 : DECOD (Ecosystem Dynamics and Sustainability), IFREMER, INRAE, Institut Agro, Plouzané, France
5 : UMR CNRS 5805 EPOC–OASU, Station Marine d’Arcachon, Université de Bordeaux, Arcachon, France
6 : IFREMER, RDT-LHYMAR, Centre Bretagne-ZI de la Pointe du Diable, Plouzané, France
Source Limnology and Oceanography methods (0024-3590) (ASLO), 2023-11 , Vol. 21 , N. 11 , P. 718-733
DOI 10.1002/lom3.10577
Abstract

Zooplankton analysis represents a bottleneck in marine ecology studies due to the difficulty to obtain zooplankton data. The last decades have seen the intense development of zooplankton imaging systems, to increase the zooplankton data spatio-temporal resolution as well as enabling the combination of size, taxonomy, and functional traits in aquatic ecology studies. Here, we propose a benchmark between the ZooScan, a commercially available, laboratory-based scanner, which analyses zooplankton preserved samples, and the ZooCAM, an in-flow imaging system designed for on-board live zooplankton imaging. Sixty-one zooplankton samples collected over the Bay of Biscay in environments ranging from estuarine to offshore blue waters were imaged with both instruments. Zooplankton Normalized Biovolume-Size Spectra slopes, mean sizes, abundances, and zooplankton community biogeographical patterns were computed for each instrument and compared at the taxonomic group, the sampling stations and the Bay of Biscay scales. Both instruments produced similar zooplankton variables by stations and by taxa and described similar zooplankton community compositions and biogeographical patterns, on the large mesozooplankton size range, i.e., [0.3–3.39] mm ESD. We conclude that the ZooCAM and the ZooScan data can be combined to generate long term or spatially resolved zooplankton time series. Our study shows that benchmarking imaging instruments or techniques (1) offers a robust assessment of interoperability between instruments, mitigating possible instrumental biases, and (2) may be of great interest in the case of instrumental obsolescence or breakdown, to choose the most conservative replacement solution in a long term time series framework

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Figure S1. Maps of the NB-SS slopes calculated by stations from (a) the ZooScan data and (b) the ZooCAM data, in the [0.3–3.39] mm ESD size range. Open symbols: stations for which NB-SS slopes were .. 540 KB Open access
Figure S2. Boxplots of the ratios computed to detect stations for which values were significantly different between the ZooScan and the ZooCAM data. Outliers indicate stations with an abnormally ... 48 KB Open access
Figure S3. Boxplots of the ratios computed to detect taxa for which values were significantly different between the ZooScan and the ZooCAM .... 47 KB Open access
Figure S4. Maps of the mean sizes as Equivalent Spherical Diameter (mm) calculated by stations from (a) the ZooScan data and (b) the ZooCAM data, in the [0.3–3.39] mm ESD size range. Open symbols:... 598 KB Open access
Figure S5. Maps of the total abundances (number of individuals.m−3) calculated by stations from (a) the ZooScan data and (b) the ZooCAM .... 584 KB Open access
Figure S6. (a) Total biovolumes (mm3) calculated for the 61 sampling stations with the ZooScan (y-axis) and the ZooCAM (x-axis). The gray line represent y = x. The black line show the linear ... 443 KB Open access
Figure S7. Total biovolumes (mm3) calculated for the 27 taxonomic groups with the ZooScan data (y-axis) and the ZooCAM data (x-axis), in ... 101 KB Open access
Figure S8. (a – c) Zooplankton community spatial structure derived from the hierarchical clustering of grid cells' coordinates in the biovolumes Principal Component Analyses (PCA) factorial space ... 941 KB Open access
Figure S9. Supplementary Materials 5 224 KB Open access
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How to cite 

Grandremy Nina, Dupuy Christine, Petitgas Pierre, Le Mestre Sophie, Bourriau Paul, Nowaczyk Antoine, Forest Bertrand, Romagnan Jean-Baptiste (2023). The ZooScan and the ZooCAM zooplankton imaging systems are intercomparable: A benchmark on the Bay of Biscay zooplankton. Limnology and Oceanography methods, 21(11), 718-733. Publisher's official version : https://doi.org/10.1002/lom3.10577 , Open Access version : https://archimer.ifremer.fr/doc/00855/96671/