Microbial Communities of the Shallow-Water Hydrothermal Vent Near Naples, Italy, and Chemosynthetic Symbionts Associated With a Free-Living Marine Nematode

Type Article
Date 2020-08
Language English
Author(s) Bellec Laure1, 2, 3, Cambon-Bonavita Marie-AnneORCID2, Durand Lucile2, Aube JohanneORCID2, Gayet Nicolas1, Sandulli Roberto4, Brandily Christophe1, Zeppilli DanielaORCID1
Affiliation(s) 1 : Ifremer, Centre Brest, REM/EEP/LEP, ZI de la Pointe du Diable, CS10070, Plouzané, France
2 : Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, CNRS, Univ Brest, Plouzané, France
3 : EPOC, UMR 5805, University of Bordeaux, Arcachon, France
4 : Laboratory of Marine Ecology, Department of Science and Technology, University of Naples “Parthenope,” Naples, Italy
Source Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2020-08 , Vol. 11 , P. 2023 (16p.)
DOI 10.3389/fmicb.2020.02023
WOS© Times Cited 11
Keyword(s) nematode, shallow-water hydrothermal vent, sulfur-oxidizing bacteria, iron cycle, Zetaproteobacteria

Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities’ investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to improve our overall understanding of these ecosystems. We used a multidisciplinary-approach, based on microscopic observations (scanning electron microscopy: SEM and Fluorescence In Situ Hybridization: FISH) coupled with a molecular diversity analysis using metabarcoding, based on the 16S rRNA gene (V3-V4 region), to characterize the bacterial community of a free-living marine nematode and its environment, the shallow hydrothermal vent near Naples (Italy). Observations of living bacteria in the intestine (FISH), molecular and phylogenetic analyses showed that this species of nematode harbors its own bacterial community, distinct from the surrounding sediment and water. Metabarcoding results revealed the specific microbiomes of the sediment from three sites of this hydrothermal area to be composed mainly of sulfur oxidizing and reducing related bacteria.

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Publisher's official version 16 6 MB Open access
Table 1 15 KB Open access
Table 2 14 KB Open access
Table 3 15 KB Open access
Table 4 16 KB Open access
Table 5 301 KB Open access
Table 6 10 KB Open access
Table 7 19 KB Open access
Table 8 39 KB Open access
Data Sheet 30 MB Open access
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Bellec Laure, Cambon-Bonavita Marie-Anne, Durand Lucile, Aube Johanne, Gayet Nicolas, Sandulli Roberto, Brandily Christophe, Zeppilli Daniela (2020). Microbial Communities of the Shallow-Water Hydrothermal Vent Near Naples, Italy, and Chemosynthetic Symbionts Associated With a Free-Living Marine Nematode. Frontiers In Microbiology, 11, 2023 (16p.). Publisher's official version : https://doi.org/10.3389/fmicb.2020.02023 , Open Access version : https://archimer.ifremer.fr/doc/00643/75544/